The spectrometer is a very important precision optical gauge for basic physical experiment,which iswidely used in some comprehensive experiments, such as monochromatic wavelength measurement, solidrefractive index measurement and ultrasonic grating measurement of sound velocity. The proficiency otregulating spectrometer can reflect students’ basic experimental skills.In practice ,due to the complex structureof the spectrometer, especially for junior undergraduates, students often encounter many difficulties in theoperation process,most students can not successfully complete the adjustment within 30 minutes.In order toachieve accurate teaching of spectrometer adjustment experiments ,the most advanced eye-movement apparatuscan be used to carry out the experimental research.Specifically , the adjustment of the spectrometer is mainlydivided into three stages:the eyepiece adjustment,the telescope optical axis direction adjustment and parallellight tube adjustment,The eye-movement apparatus are used to get the operator to look at a heat map, trackdiagram and the eye movement data, in order to conclude the difficulties of the three stages and analyze theadjustment characteristics of each stage. By comparing the fixation characteristics of undergraduates andpostgraduates in trial experiment, formal experiment and guided experiment, the problems existing in theadjustment of spectrometer are found,and the learning characteristics of undergraduates and postgraduates arecompared,thus guidance is provided for the establishment of appropriate experimental teaching methods.