The ideological and political content mining is the foundation of the ideological and political curriculum．Combining with the characteristics of the course of“University physics experiment”，it explores the humanistic spirit of the course from four aspects: patriotic spirit of scientists，noble personality，rational and scientific sentiment for truth，and professional ethics．Thus，it is not only beneficial to improve the interesting and ideological nature of the class，but also to enrich students’political spirit and build a complete and noble personality．