In the Millikan oil drop experiment，the measurement of oil droplet falling time and equilibrium voltage constitutes two primary factors influencing experimental results． To address the issue of insufficient precision in measuring oil droplet descent time during the experiment，this study employs the Tracker software to track the falling trajectories and timing of oil droplets，thereby enhancing the measurement accuracy of descent time．During specific experimental procedures，we simultaneously utilized traditional manual timing and Tracker software tracking technology to measure the descent times of 20 oil droplets． Comparative analysis revealed that the Tracker-based tracking method improves the precision of oil droplet falling time measurements to the 0．001 s level，significantly enhancing temporal resolution．This advancement consequently yields more accurate charge quantity measurements．Furthermore，the Tracker software＇s visualization of droplet trajectories provides intuitive demonstration of Brownian motion effects on microscopic oil droplets．