Under the perspective of“Curriculum Ideology and Politics”，petroleum spirit is a unique cultural spirit advocated by China’s petroleum industry．Integrating petroleum spirit into university physics experiment course is helpful to cultivate students’spirit of innovation，teamwork and truth-seeking． It analyzes the limitations of college physics experiment courses，combines the value and significance of petroleum spirit，puts forward a series of methods and strategies to integrate petroleum spirit into college physics experiment courses effectively，realizes the combination of teaching research and practice extension，strengthens the teaching quality of college physics experiment courses，and improves students’comprehensive hands-on and innovative abilities．It is of great significance to promote the reform of university physics experiment teaching．