Due to the fact that the textbook is only equipped with the instrument plan，the instrument structure function introduction and adjustment description，it is difficult for students to master the principle，structure and adjustment of the instrument in the limited class time，especially for the equipment with relatively complex structure，so the use and learning of experimental instruments has become a difficulty in experimental teaching．To solve this problem，with the core function of“getting familiar with instrument structure，mastering instrument principle and learning instrument operation”，three-dimensional animation of experimental instruments is designed and developed by using CINEMA 4D and applied in teaching．Through animation，students can better learn the internal and external structure，principle and adjustment of the instrument．It makes up for students’ lack of spatial thinking effectively and improves the effect of physics experiment teaching．