In order to improve the physics core literacy of middle school and university students，and solve the problem of the connection between middle school and university physics teaching，the methods of investigation and induction are adopted to analyze the physics experiments between middle school and university and explore the existing connection problems．From the three levels of middle school，university and education management department，it puts forward countermeasures around teaching concept，teaching content and students ’ experimental ability，such as adjusting educational concept，regularizing physics experiment course in middle school，reforming the teaching method of physics experiment in university，and establishing the connecting

system of physical experiment skill cultivation． Expand the experiment content，the middle school physics experiment into the project learning and other modern teaching mode，university based on scientific research laboratory open to basic physics experiment，strengthen the provincial primary and secondary school teachers professional development training project; Create experimental conditions，strengthen the construction of basic laboratories in middle schools，open preparatory experiments in universities，establish online virtual simulation laboratories．We can improve the students’physical experiment ability and scientific literacy through the reform of multi-layer physical experiment，and effectively connect the physical experiment of experimental middle school and university．