ln order to stimulate students' interest in seientifie research ,eultivate innovative talents ,and closelyfollow the seientifie frontier of nanotechnology, a comprehensive experimental design based on opticalinterference effect and contrast imaging to characterize the layer-number distribution of two-dimensionalmaterials was designed. In the experiment, large-area single-layer graphene films with multi-ayer crystaldomains were selected, images were colleeted by oplical microseope, and the number and distribution ofgraphene layers were analyzed and characterized by optieal contrast imaging.Furthermore , the contrast weightsof the three primary color components are superimposed by the gray value , and the high-resolution contrastimaging ean be achieved with high precision layer number and grain boundary identifieation.The influenee ofoplical interference effect on optical contrast is explored with this example.ln the course of the experiment,students’ understanding of new two-dimensional semiconductor materials and the interierence eleet of lichtwere enhaneed.Students' hands-on ability was eultivated through the operation of optieal mieroscope ,and theirseientifie thinking ability was eultivated through data fiting and analysis.lt integrates the seientifie frontier withthe experimental teaehing, and promoles the innovation, interesls and praelicability of the experiment. lt isbenefeial to cultivate students’ practical ability ,seientife aecomplishment and innovation ability.