Multistage amplifier circuit is an important teaching content in the course of fundamentals of electronics. In this paper, a typical direct coupling multistage amplifier circuit was analyzed by using theoretical calculation and Multisim simulation, and the positive role of Multisim simulation played in the teaching of the course of fundamentals of electronics was highlighted. Multisim simulation can show the abstract and boring teaching content vividly, which can improve the learning interest of college students. The teaching methodology combining theoretical analysis and Multisim simulation is helpful for college students to better understand and master the teaching content, and then improve the teaching quality and get the expected teaching results.