基于改进型迈克尔逊干涉仪的定量相位显微技术

doi:10.14139/j.cnki.cn22-1228.2022.03.015

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摘要

数字全息技术可以记录目标物体的相位，进行无标记成像。通过并行偏振像移全息技术与迈克尔逊干涉仪结合，利用偏振相机与波片改进同轴迈克尔逊式全息显微镜，实现了单帧定量复场成像，动态成像和显微成像。实验结果表明，该方法可以单帧恢复物体的复场信息，可以动态成像，并且对小鼠肝细胞进行无标记、无接触定量相位成像。

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	杜兴鹏
	陈垲全
	刘汉子
	朱浩天
	陈子阳

关键词: 数字全息术复场成像动态成像显微成像偏振像移迈克尔逊干涉仪

Abstract:

Digital holography can record the phase of specimen，which can be applied in label-free imaging．Combined parallel polarization phase shifting techniques with Michelson interferometer，the on-axis Michelson-type holographic microscopy is optimized by using polarization camera and wave plate． Quantitative complexfield imaging，dynamic imaging and single-shot microscopic imaging are realized by the proposed system．Experimental results show that this method can snapshot reconstruct complex field information of the object，dynamic imaging and unlabeled quantitative phase imaging for the mouse liver cells．

Key words: digital holography complex field imaging dynamic imaging microscopic imaging polarization image-shifting michelson interferometer

出版日期: 2022-06-25 发布日期: 2022-06-25 整期出版日期: 2022-06-25

ZTFLH:

O 4-33

引用本文:

杜兴鹏, 陈垲全, 刘汉子, 朱浩天, 陈子阳. 基于改进型迈克尔逊干涉仪的定量相位显微技术 [J]. 大学物理实验, 2022, 35(3): 71-74.
DU Xingpeng, CHEN Kaiquan, LIU Hanzi, ZHU Haotian, CHEN Ziyang. Quantitative Phase Microscopy Based on the Modified Michelson Interferometer . Physical Experiment of College, 2022, 35(3): 71-74.

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http://dawushiyan.jlict.edu.cn/CN/10.14139/j.cnki.cn22-1228.2022.03.015 或 http://dawushiyan.jlict.edu.cn/CN/Y2022/V35/I3/71

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