It designs and implements an experimental simulation software for ultrasonic sound speed measurement based on Matlab GUI. The software consists of four interfaces,login,welcome,experimenta content and experimental summary.Among them,the experimental content interface includes four functiona modules:experimental principle,video explanationstanding wave method measurement experiment and phase comparison method measurement experiment,while the measurement experimental interfaces of standing wave method and phase comparison method all consist of five parts: graphical display, parameter setting, data recording,data processing and error analysisThe simulation software has the advantages of complete function low use cost,intuitive image,simple operationuser-friendly interface,flexible and controllable parameter setting,and fast and accurate data processing.It can not only stimulate students’interest and enthusiasm in the experiment,but also help students to deeplyunderstand the experimental principle and enhance the effect of the experiment teaching.