Based on the background of engineering education certification and the current teaching situation of biochemistry experiments, the goal of experimental teaching courses to support graduation requirements is designed. With the help of the "Chaoxing learning platform", the mixed online and offline teaching mode reform is carried out for the biochemistry experiment courses. The mixed online and offline teaching has practically improved the teaching quality of biochemistry experiment courses, effectively supported the achievement of course goals and graduation requirements in engineering education certification, and improved students' ability to solve complex engineering problems.